Keap1/Nrf2/HO-1 通路在丹皮酚保护小鼠重症急性胰腺炎 中的作用及机制研究

Abstract

(Objective) To investigate the effect of paeonol on oxidative damage in mice with acute pancreatitis. (Method) In this study, a mouse model of acute pancreatitis was established by injected intraperitoneally with 20% L-arginine, and the experimental animals were randomly assigned to the blank control group acute pancreatitis model group and the paeonol low-medium-high dose group (25, 50, 100 mg/kg∙bw). Except blank control group, the other 4 groups were injected intraperitoneally with 20% L-arginine after 5 days of normal gavage of paeonol, and serum of mice were taken to measure the oxidation index (MDA SOD) 6 hours later, and pancreatic tissues of mice were taken to observe the pathological changes; At the same time, immunohistochemical sections of pancreatic tissue MPO were made to observe the histopathological changes, the distribution and activity of MPO. The mRNA expression levels of HO-1、Keap1 and Nrf2 in pancreatic tissues of each group were detected by fluorescence quantitative PCR, and the protein expression levels of HO-1、Keap1 and Nrf2 were detected by Western blot. Conclusion: Paeonol may alleviate oxidative damage induced by Larginine in mice with acute pancreatitis by activating Keap1/Nrf2/HO-1 signaling pathway. This study provides theoretical reference for the pathogenesis of acute pancreatitis and the development of related drugs.